![Molecular Biology Laboratory [ddPCR] International Workshop](https://tiu.edu.iq/workshops/wp-content/uploads/2021/04/Logo.png){kind=logo}
Department: Medical Analysis
The workshop in brief: The primary goal of the Molecular Biology Laboratory, equipped with digital droplet PCR (ddPCR) technology, is to advance research, diagnostics, and training in the field of molecular biology. ddPCR provides highly sensitive and accurate quantification of nucleic acids, enabling breakthroughs in disease diagnostics, genetic research, and environmental studies. The laboratory also aims to enhance collaboration between academia and the biotechnology sector by fostering skill development and innovation.
Participants: Between 80 – 90 participants
Place: Tishk International University_ Seminar Hall no. 302
Session 1_ The impact of positive thinking on students’
learning potential.
Session 1_
“Digital PCR: A Revolutionary Approach to Nucleic Acid Quantification,” where Dr. Ayman talked about the principles, applications, and advantages of using the Digital Droplet PCR. He mentioned that digital PCR (dPCR) is an advanced molecular technique for quantifying nucleic acids. It improves upon conventional PCR by offering absolute quantification instead of relative results. The key principle of dPCR is partitioning the sample into thousands of droplets or wells. Each partition undergoes independent amplification, enhancing sensitivity and precision. Detection is achieved by measuring fluorescent signals from each partition. Unlike traditional methods, dPCR eliminates the need for standard curves. It is highly resistant to inhibitors, making it robust across various samples. dPCR is particularly useful for detecting rare genetic variants.
Session 2_
Dr. Mukhlis’s presentation was about an “Overview of Molecular Diagnostics Laboratory Techniques.” He mentioned that molecular diagnostics laboratory techniques are essential tools for analyzing biological markers in the genome and proteome. They focus on DNA, RNA, and protein detection to diagnose and monitor diseases accurately. Polymerase Chain Reaction (PCR) is widely used for amplifying DNA and detecting specific genetic material. Real-time PCR adds quantitative analysis through fluorescence signals. Another technique, fluorescence in situ hybridization (FISH), identifies chromosomal abnormalities and gene rearrangements.
Speaker 1: Dr. Ayman Aldabsheh, the General Manager of Al Rayyan International Scientific Company (RISC) and a biotechnologist and genetics engineer with over 20 years’ of experience in the field of human, pharmaceutical, veterinary, and agricultural molecular biology applications (genomics and proteomics).
Speaker 2: Dr. Mukhlis Hamad, an expert in human molecular genetics in the Erbil City-Kurdistan Region of Iraq. He completed a PhD in Human Molecular Genetics in 2018 through a joint program between Salahaddin University-Erbil (SUE) and University Technology Malaysia (UTM).
Outcomes of the Workshop:
The workshop on Molecular Biology and ddPCR technology resulted in several key achievements:
1. Technical Training: Participants were introduced to the principles and practical applications of ddPCR, including its use in gene expression analysis, mutation detection, and copy number variation studies.
2. Skill Development: Hands-on sessions allowed attendees to gain practical experience with ddPCR equipment, improving their technical proficiency in molecular diagnostics.
3. Collaborative Opportunities: The event facilitated networking among researchers, clinicians, and industry experts, paving the way for joint projects and research initiatives.
4. Promotion of Advanced Techniques: Awareness was raised about the advantages of ddPCR over conventional PCR methods, emphasizing its precision and robustness in various fields of application.
5. Capacity Building: Participants discussed strategies to integrate ddPCR into routine diagnostics and research, particularly in areas of healthcare and environmental monitoring.
